Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs
نویسندگان
چکیده
منابع مشابه
Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Translational stop codon readthrough provides a regulatory mechanism of gene expression that is extensively utilised by positive-sense ssRNA viruses. The misreading of termination codons is achieved by a variety of naturally occurring suppressor tRNAs whose structure and function is the subject of this survey. All of the nonsense suppressors characterised to date (with the exception of selenocy...
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Codon nonsense mutations include amber, ochre, or opal mutations according to termination codon consisting of three types (TAG, TAA and TGA). Codon nonsense mutations are also divided into natural and artificial mutations. We discussed the interaction of codon nonsense mutations and suppressor tRNAs in vitro and in vivo. Nonsense suppressions do not only happen in prokaryotes but also in eukary...
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It is known from experiments with bacteria and eukaryotic viruses that readthrough of termination codons located within the open reading frame (ORF) of mRNAs depends on the availability of suppressor tRNA(s) and the efficiency of termination in cells. Consequently, the yield of readthrough products can be used as a measure of the activity of polypeptide chain release factor(s) (RF), key compone...
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The universal genetic code includes three codons which signal polypeptide chain termination. These termination or nonsense codons are UAG (amber), UAA (ochre), and UGA (opal). Usually, Escherichia coli and other procaryotic cells do not contain transfer ribonucleic acids (tRNAs) which recognize these codons. However, such tRNAs can be created by suppressor mutations in tRNA genes, generating tR...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2001
ISSN: 1362-4962
DOI: 10.1093/nar/29.23.4767